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Registro Completo |
Biblioteca(s): |
Embrapa Pantanal. |
Data corrente: |
19/07/2023 |
Data da última atualização: |
19/07/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
NOGUEIRA, E.; TIRPÁK, F.; HAMILTON, L.; ZIGO, M.; KERNS, K.; SUTOVSKY, M.; KIM, J.; VOLKMANN, D.; JOVINE, L.; TAYLOR, J. F.; SCHNABEL, R. D.; SUTOVSKY, P. |
Afiliação: |
ERIKLIS NOGUEIRA, CPAP; FILIP TIRPÁK, Slovak University of Agriculture; LAUREN HAMILTON, UNIVERISTY OF MISSOURI; MICHAL ZIGO, UNIVERSITY OF MISSOURI; KARL KERNS, UNIVERSITY OF MISSOURI; MIRIAM SUTOVSKY, UNIVERSITY OF MISSOURI; JAEWOO KIM, UNIVERSITY OF MISSOURI; DIETRICH VOLKMANN, UNIVERSITY OF MISSOURI; LUCA JOVINE, KAROLINSKA INSTITUTET; JEREMY F. TAYLOR, UNIVERSITY OF MISSOURI; ROBERT D. SCHNABEL, UNIVERSITY OF MISSOURI; PETER SUTOVSKY, UNIVERSITY OF MISSOURI. |
Título: |
A Non-synonymous point mutation in a WD-40 domain repeat of EML5 leads to decreased bovine sperm quality and fertility. |
Ano de publicação: |
2022 |
Fonte/Imprenta: |
Frontiers in Cell and Developmental Biology, v. 10, 872740, Apr. 2022. |
ISSN: |
2296-634X |
DOI: |
https://doi.org/10.3389/fcell.2022.872740 |
Idioma: |
Inglês |
Conteúdo: |
This study is part of a concerted effort to identify and phenotype rare, deleterious mutations that adversely affect sperm quality, or convey high developmental and fertility potential to embryos and ensuing progeny. A rare, homozygous mutation in EML5 (EML5R1654W), which encodes a microtubule-associated protein with high expression in testis and brain was identified in an Angus bull used extensively in artificial insemination (AI) for its outstanding progeny production traits. The bull?s fertility was low in cross-breeding timed AI (TAI) (Pregnancy/TAI = 25.2%; n = 222) and, in general, AI breeding to Nellore cows (41%; n = 822). A search of the 1,000 Bull Genomes Run9 database revealed an additional 74 heterozygous animals and 8 homozygous animals harboring this exact mutation across several different breeds (0.7% frequency within the 6,191 sequenced animals). Phenotypically, spermatozoa from the homozygous Angus bull displayed prominent piriform and tapered heads, and outwardly protruding knobbed acrosomes. Additionally, an increased retention of EML5 was also observed in the sperm head of both homozygous and heterozygous Angus bulls compared to wild-type animals. This non-synonymous point mutation is located within a WD40 signaling domain repeat of EML5 and is predicted to be detrimental to overall protein function by genomic single nucleotide polymorphism (SNP) analysis and protein modeling. Future work will examine how this rare mutation affects field AI fertility and will characterize the role of EML5 in spermatogenesis. MenosThis study is part of a concerted effort to identify and phenotype rare, deleterious mutations that adversely affect sperm quality, or convey high developmental and fertility potential to embryos and ensuing progeny. A rare, homozygous mutation in EML5 (EML5R1654W), which encodes a microtubule-associated protein with high expression in testis and brain was identified in an Angus bull used extensively in artificial insemination (AI) for its outstanding progeny production traits. The bull?s fertility was low in cross-breeding timed AI (TAI) (Pregnancy/TAI = 25.2%; n = 222) and, in general, AI breeding to Nellore cows (41%; n = 822). A search of the 1,000 Bull Genomes Run9 database revealed an additional 74 heterozygous animals and 8 homozygous animals harboring this exact mutation across several different breeds (0.7% frequency within the 6,191 sequenced animals). Phenotypically, spermatozoa from the homozygous Angus bull displayed prominent piriform and tapered heads, and outwardly protruding knobbed acrosomes. Additionally, an increased retention of EML5 was also observed in the sperm head of both homozygous and heterozygous Angus bulls compared to wild-type animals. This non-synonymous point mutation is located within a WD40 signaling domain repeat of EML5 and is predicted to be detrimental to overall protein function by genomic single nucleotide polymorphism (SNP) analysis and protein modeling. Future work will examine how this rare mutation affects field AI fertility and ... Mostrar Tudo |
Thesagro: |
Espermatozóide; Fertilidade Animal. |
Thesaurus Nal: |
Acrosome; Animal fertility; Biogenesis; Fertilization (reproduction); Spermatozoa; Spermiogenesis. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1155065/1/fcell-2022.pdf
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Marc: |
LEADER 02641naa a2200373 a 4500 001 2155065 005 2023-07-19 008 2022 bl uuuu u00u1 u #d 022 $a2296-634X 024 7 $ahttps://doi.org/10.3389/fcell.2022.872740$2DOI 100 1 $aNOGUEIRA, E. 245 $aA Non-synonymous point mutation in a WD-40 domain repeat of EML5 leads to decreased bovine sperm quality and fertility.$h[electronic resource] 260 $c2022 520 $aThis study is part of a concerted effort to identify and phenotype rare, deleterious mutations that adversely affect sperm quality, or convey high developmental and fertility potential to embryos and ensuing progeny. A rare, homozygous mutation in EML5 (EML5R1654W), which encodes a microtubule-associated protein with high expression in testis and brain was identified in an Angus bull used extensively in artificial insemination (AI) for its outstanding progeny production traits. The bull?s fertility was low in cross-breeding timed AI (TAI) (Pregnancy/TAI = 25.2%; n = 222) and, in general, AI breeding to Nellore cows (41%; n = 822). A search of the 1,000 Bull Genomes Run9 database revealed an additional 74 heterozygous animals and 8 homozygous animals harboring this exact mutation across several different breeds (0.7% frequency within the 6,191 sequenced animals). Phenotypically, spermatozoa from the homozygous Angus bull displayed prominent piriform and tapered heads, and outwardly protruding knobbed acrosomes. Additionally, an increased retention of EML5 was also observed in the sperm head of both homozygous and heterozygous Angus bulls compared to wild-type animals. This non-synonymous point mutation is located within a WD40 signaling domain repeat of EML5 and is predicted to be detrimental to overall protein function by genomic single nucleotide polymorphism (SNP) analysis and protein modeling. Future work will examine how this rare mutation affects field AI fertility and will characterize the role of EML5 in spermatogenesis. 650 $aAcrosome 650 $aAnimal fertility 650 $aBiogenesis 650 $aFertilization (reproduction) 650 $aSpermatozoa 650 $aSpermiogenesis 650 $aEspermatozóide 650 $aFertilidade Animal 700 1 $aTIRPÁK, F. 700 1 $aHAMILTON, L. 700 1 $aZIGO, M. 700 1 $aKERNS, K. 700 1 $aSUTOVSKY, M. 700 1 $aKIM, J. 700 1 $aVOLKMANN, D. 700 1 $aJOVINE, L. 700 1 $aTAYLOR, J. F. 700 1 $aSCHNABEL, R. D. 700 1 $aSUTOVSKY, P. 773 $tFrontiers in Cell and Developmental Biology$gv. 10, 872740, Apr. 2022.
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Registro original: |
Embrapa Pantanal (CPAP) |
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Registros recuperados : 25 | |
5. | | NOGUEIRA, E.; TIRPÁK, F.; HAMILTON, L.; ZIGO, M.; KERNS, K.; SUTOVSKY, M.; KIM, J.; VOLKMANN, D.; JOVINE, L.; TAYLOR, J. F.; SCHNABEL, R. D.; SUTOVSKY, P. A Non-synonymous point mutation in a WD-40 domain repeat of EML5 leads to decreased bovine sperm quality and fertility. Frontiers in Cell and Developmental Biology, v. 10, 872740, Apr. 2022.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
Biblioteca(s): Embrapa Pantanal. |
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6. | | SOUZA, M. M. de; ZERLOTINI NETO, A.; GEISTLINGER, L.; TIZIOTO, P. C.; TAYLOR, J. F.; ROCHA, M. I. P.; DINIZ, W. J. S.; COUTINHO, L. L.; REGITANO, L. C. de A. A comprehensive manually-curated compendium of bovine transcription factors. Scientific Reports, v. 8, p. 1-12, 2018. Article number: 13747. Na publicação: Adhemar Zerlotini, Luciana C. A. Regitano.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Agricultura Digital; Embrapa Pecuária Sudeste. |
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7. | | ROFL, M. M.; DECKER, J. E.; McKAY, S. D.; TIZIOTO, P. C.; BRANHAM, K. A.; WHITACRE, L. K.; HOFF, J. L.; REGITANO, L. C. de A.; TAYLOR, J. F. Genomics in the United States beef industry. Livestock Science, v.166, p. 84-93, aug. 2014.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
Biblioteca(s): Embrapa Pecuária Sudeste. |
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8. | | BEHURA, S. K.; TIZIOTO, P. C.; KIM, J.; GRUPIONI, N. V.; SEABURY, C. M.; SCHNABEL, R. D.; GERSHWIN, L. J.; VAN EEnenNAAM, A. L.; TOAF-FROSENSTEIN, R.; NEIBERGS, H. L.; REGITANO, L. C. de A.; TAYLOR, J. F. Tissue tropism in host transcriptional response to members of the bovine respiratory disease complex. Scientific Reports, v.7, n. 17938, p. 1-15, 2017.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Pecuária Sudeste. |
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9. | | TIZIOTO, P. C.; COUTINHO, L. L.; GASPARIN, G.; MALAGO JUNIOR, W.; DONATONI, F. A. B.; TULLIO, R. R.; NASSU, R. T.; TAYLOR, J. F.; REGITANO, L. C. de A. Variation in myogenic differentiation 1 mRNA abundance is associated with beef tenderness in Nelore cattle. Animal Genetics, v.47, n.4, p. 491-494, aug. 2016. 47 491-494Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
Biblioteca(s): Embrapa Pecuária Sudeste. |
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10. | | TIZIOTO, P.; COUTINHO, L. L.; DECKER, J. E.; SCHNABEL, R. D.; ROSA, C. O.; OLIVEIRA, P. S. N.; SOUZA, M. M.; MOURÃO, G. B.; TULLIO, R. R.; CHAVES, A. S.; LANNA, D. P. D.; ZERLOTINI NETO, A.; MUDADU, M. A.; TAYLOR, J. F.; REGITANO, L. C. A. Global liver gene expression differences in Nelore steers with divergent residual feed intake phenotypes. BMC Genomics, London, v. 16, p. 1-14, 2015.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Agricultura Digital. |
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11. | | TIZIOTO, P.; COUTINHO, L. L.; DECKER, J. E.; SCHNABEL, R. D.; ROSA, K. O.; OLIVEIRA, P. S. N.; SOUZA, M. M.; MOURÃO, G. B.; TULLIO, R. R.; CHAVES, A. S.; LANNA, D. P. D.; ZERLOTINI NETO, A.; MUDADU, M. de A.; TAYLOR, J. F.; REGITANO, L. C. de A. Global liver gene expression differences in Nelore steers with divergent residual feed intake phenotypes. BMC Genomics, v. 16, n. 242, 2015.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Pecuária Sudeste. |
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12. | | TIZIOTO, P. L.; COUTINHO, L. L.; OLIVEIRA, P. S. N.; CESAR, A. S. M.; DINIZ, W. J. S.; LIMA, A. O.; ROCHA, M. I.; DECKER, J. E.; SCHNABEL, R. D.; MOURÃO, G. B.; TULLIO, R. R.; ZERLOTINI NETO, A.; TAYLOR, J. F.; REGITANO, L. C. de A. Gene expression differences in Longissimus muscle of Nelore steers genetically divergent for residual feed intake. Scientific Reports, v. 6, p. 1-12, 2016.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Agricultura Digital; Embrapa Pecuária Sudeste. |
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13. | | TIZIOTO, P. C.; TAYLOR, J. F.; DECKER, J. E.; GROMBONI, C. F.; MUDADU, M. de A.; SCHNABEL, R. D.; COUTINHO, L. L.; MOURÃO, G. B.; NASSU, R. T.; DONATONI, F. A. B.; THOLON, P.; SONSTEGARD, T. S.; ALENCAR, M. M. de; TULLIO, R. R.; REECY, J. M.; NOGUEIRA, A. R. de A.; REGITANO, L. C. de A. Effects of quantitative trait loci on iron content of bovine longissimus dorsi muscle. In: WORLD CONGRESS OF GENETICS APPLIED TO LIVESTOK PRODUCTION, 10., 2014, Vancouver. Proceedings... Vancouver: American Society of Animal Science, 2014. 3 p.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Pecuária Sudeste. |
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14. | | DECKER, J. E.; MCKAY, S. D.; ROLF, M. M.; ALCALA, A. M.; SONSTEGARD, T. S.; HANOTTE, O.; GOTHERSTROM, A.; SEABURY, C. M.; PRAHARANI, L.; BABAR, M. E.; REGITANO, L. C. de A.; YILDIZ, M. A.; HEATON, M. P.; LIU, W. S.; LEI, C. Z.; REECY, J. M.; SAIF-UR-REHMAN, M.; SCHNABEL, R. D.; TAYLOR, J. F. Worldwide patterns of ancestry, divergence, and admixture in domesticated cattle. Plos Genetics, v. 10, n. 3, e1004254, 2014. 14 p.Tipo: Artigo em Periódico Indexado |
Biblioteca(s): Embrapa Pecuária Sudeste. |
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15. | | TIZIOTO, P. C.; DECKER, J. E.; TAYLOR, J. F.; SCHNABEL, R. D.; MUDADU, M. de A.; MOURAO, G. B.; COUTINHO, L. L.; THOLON, P.; SONSTEGARD, T. S.; ROSA, A. do N.; ALENCAR, M. M. de; TULLIO, R. R.; MEDEIROS, S. R. de; NASSU, R. T.; FEIJO, G. L. D.; SILVA, L. O. C. da; TORRES JUNIOR, R. A. de A.; SIQUEIRA, F.; HIGA, R. H.; REGITANO, L. C. de A. Genome scan for meat quality traits in Nelore beef cattle. Physiological genomics, v. 45, n. 21, p. 1012-1020, 2013.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
Biblioteca(s): Embrapa Pecuária Sudeste. |
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16. | | TIZIOTO, P. C.; DECKER, J. E.; TAYLOR, J. F.; SCHNABEL, R. D.; MUDADU, M. de A.; SILVA, F. L.; MOURÃO, G. B.; COUTINHO, L. L.; THOLON, P.; SONSTEGARD, T. S.; ROSA, A. do N.; ALENCAR, M. M. de; TULLIO, R. R.; MEDEIROS, S. R. de; NASSU, R. T.; FEIJO, G. L. D.; SILVA, L. O. C. da; TORRES, R. A.; SIQUEIRA, F.; HIGA, R. H.; REGITANO, L. C. de A. Genome scan for meat quality traits in Nelore beef cattle. Physiological Genomics, v. 45, n. 21, p. 1012-1020, September 2013.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
Biblioteca(s): Embrapa Agricultura Digital; Embrapa Gado de Corte. |
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17. | | TIZIOTO, P. C.; DECKER, J. E.; SCHNABEL, R. D.; MUDADU, M. de A.; SILVA, F. L.; MOURÃO, G. B.; COUTINHO. L. L.; THOLON, P.; SONSTEGARD, T. S; ROSA, A. do N.; ALENCAR, M. M. de; TULLIO, R. R.; MEDEIROS, S. R.; NASSU, R. T.; FEIJO, G. L. D.; SILVA, L. O. C. da; SIQUEIRA, F.; HIGA, R. H.; TAYLOR, J. F.; REGITANO, L. C. de A. Genome scan revealed new pathways related to meat tenderness in Nelore beef cattle. In: INTERNATIONAL SYMPOSIUM ON ANIMAL FUNCTIONAL GENOMICS, 5., 2013, Guarujá. Abstract... Guarujá:[ s.n.], 2013. AB.08.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Pecuária Sudeste. |
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18. | | TIZIOTO, P. L.; DECKER, J. E.; SCHNABEL, R. D.; MUDADU, M. A.; SILVA, F. L.; MOURÃO, G. B.; COUTINHO, L. L.; THOLON, P.; SONSTEGARD, T. S.; ROSA, A. N.; ALENCAR, M. M.; TULLIO, R. R.; MEDEIROS, S. R.; NASSU, R. T.; FEIJÓ, G. L. D.; SILVA, L. O. C.; TORRES, R. A.; SIQUEIRA, F.; HIGA, R. H.; TAYLOR, J. F.; REGITANO, L. C. de A. Genome scan revealed new pathways related to meat tenderness in Nelore beef cattle. In: INTERNATIONAL SYMPOSIUM ON ANIMAL FUNCTIONAL GENOMICS, 5., 2013, Guarujá. Programme and abstract book... [S.l.: s.n.], 2013. p. 15. ISAFG 2013. AB.08.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Gado de Corte. |
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19. | | TIZIOTO, P. L.; DECKER, J. E.; SCHNABEL, R. D.; MUDADU, M. A.; SILVA, F. L.; MOURÃO, G. B.; COUTINHO, L. L.; THOLON, P.; SONSTEGARD, T. S.; ROSA, A. N.; ALENCAR, M. M.; TULLIO, R. R.; MEDEIROS, S. R.; NASSU, R. T.; FEIJÓ, G. L. D.; SILVA, L. O. C.; TORRES, R. A.; SIQUEIRA, F.; HIGA, R. H.; TAYLOR, J. F.; REGITANO, L. C. de A. Genome scan revealed new pathways related to meat tenderness in Nelore beef cattle. In: INTERNATIONAL SYMPOSIUM ON ANIMAL FUNCTIONAL GENOMICS, 5., 2013, Guarujá. Programme and abstract book... [S.l.: s.n.], 2013. p. 15. ISAFG 2013. AB.08.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Agricultura Digital. |
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20. | | DECKER, J. E.; PIRES, J. C.; CONANT, G. C.; MCKAY, S. D.; HEATON, M. P.; VILKKI, J.; CHEN, K.; COOPER, A.; SEABURY, C. M.; CAETANO, A. R.; JOHNSON, G. S.; BRENNEMAN, R. A.; HANOTTE, O.; COUTINHO, L. L.; BABAR, M. E.; EGGERT, L. S.; WIENER, P.; KIM, J.-J.; KIM, K. S.; SONSTEGARD, T. S.; TASSELL, C. P. van; NEIBERGS, H. L.; SCHNABEL, R. D.; TAYLOR, J. F. Divergence times and signatures of selection from phylogenomic analysis of Pecoran species. In: INTERNATIONAL PLANT & ANIMAL GENOMES CONFERENCE, 17., 2009, San Diego, CA. [Proceedings...]. [S. l.: s.n.], 2009.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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Registros recuperados : 25 | |
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Nenhum registro encontrado para a expressão de busca informada. |
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